PAH degradation under various conditions of bioavailability will be assessed as to improve rational exploitation of the catalytic properties of bacteria for the treatment and prevention of PAH pollution. We will generate a knowledge base not only on the microbial catabolome for biodegradation of PAHs in various impacted environmental settings based on genome gazing, retrieval and characterization of specific enzymes but also on systems related bioavailability of contaminant mixtures.
MAGICPAH takes into account the tremendous undiscovered meta-genomic resources by the direct retrieval from genome/meta-genome libraries and consequent characterization of enzymes through activity screens. These screens will include a high-end functional small-molecule fluorescence screening platform and will allow us to directly access novel metabolic reactions followed by their rational exploitation for bio-catalysis and the re-construction of biodegradation networks. Results from (meta-) genomic approaches will be correlated with microbial in situ activity assessments, specifically dedicated to identifying key players and key reactions involved in anaerobic PAH metabolism.
Key processes for PAH metabolism particularly in marine and composting environments and the kinetics of aerobic degradation of PAH under different conditions of bioavailability will be assessed in model systems, the rational manipulation of which will allow us to deduce correlations between system performance and genomic blueprint. The results will be used to improve treatments of PAH-contaminated sites.
PROJECT GOALS:The main objectives of MAGICPAH in the context of the above are:
i) to generate a knowledge base of the microbial aerobic catabolome with particular relevance to biodegradation of PAHs in various impacted environmental settings
ii) to develop concepts to quantify in situ degradation of PAH employing combined hydrogen and carbon stable isotope analysis
iii) to identify key players and key reactions involved in anaerobic PAH metabolism
iv) to achieve a detailed understanding on key processes for PAH metabolism in marine and composting environments
v) to develop methods to predict the ultimate fate and the kinetics of aerobic degradation of PAH under different conditions of bioavailability
vi) to isolate and sequence novel key players in PAH metabolism to understand the genomic basis of niche specificities that allow microbes to thrive and function in extreme PAH impacted environments
vii) to investigate the potential synergistic links between environmental biotechnology and medical biotechnology by assessing novel biocatalysts for their use in new biocatalytic processes.
viii) to integrate detailed catabolome and reactome information through bioinformatic techniques to re-construct metabolic networks
ix) to apply gathered information to improve the treatment performance of PAH contaminated sites
By those methods, genes coding enzymes of interest can be screened from various environments including soils, compost piles, landfill leachate, bioreactors and activated sludges, marine water and sediment samples (including tidal flat sediments, deep sea and water column) and freshwater samples (including drinking water, pond water, rivers and hot springs), to cite some
• 8 phage-?clone libraries
• 16 fosmid-?clone libraries
The origin of the libraries is DNA of microbial communities from:
• 14 distinct marine samples
• 10 distinct terrestrial samples
The MAGICPAH Consortium gets ready a set of 1200 clones containing enzyme of interest. They include those encoding the following activities:
• Rieske non-?heme iron oxygenases
• Extradiol dioxygenases
• Esterases and lipases
• meta-?cleavage product (MCP) hydrolases
• Glycosyl hydrolases
? The clones available are ready?to-use in the form of separate individual clones or pool of clones. This may help designing the most appropriate screening platform.
? In deep and/or preliminary biochemical data are available for all clones encoding activities of interest.
? The targets with relevant biotechnologically-?relevant characteristics are identified. Features such as relevance for biotechnology processes (i.e. production of enantiomerss), salt-? tolerance, temperature and pH optima and substrate profile are available.
This innovation is the result of the project
Title: Molecular Approaches And Metagenomic Investigations For Optimizing Clean-Up Of PAH Contaminated Sites
Organisations and people involved in this eco-innovation.
Please click on an entry to view all contact details.
HELMHOLTZ-ZENTRUM FUER INFEKTIONSFORSCHUNG GMBH
Role in project: Project Coordination
Contact person: Dr. CHHATWAL G. Singh
Phone: +495316181 4400
Contact person: Ms. VILSTRUP Britt Sønberg
AECOM CZ SRO
Contact person: SPISKOVA Vendulka
AGENCIA ESTATAL CONSEJO SUPERIOR DE INVESTIGACIONES CIENTIFICAS
Field: Scientific research and development (Spain)
Contact person: Mr. ABAD RUIZ Carlos Manuel
Contact person: Ms. DAVEY Christine
COMMISSARIAT A L ENERGIE ATOMIQUE ET AUX ENERGIES ALTERNATIVES
Contact person: Mr. COPIN Régis
CONSIGLIO NAZIONALE DELLE RICERCHE
Contact person: Ms. IRRERA Gaetana
Contact person: Mr. DIAZ Luis Fernando
DANMARKS TEKNISKE UNIVERSITET
Contact person: Ms. RASMUSSEN Birte Kastrup
HELMHOLTZ-ZENTRUM FUER UMWELTFORSCHUNG GMBH - UFZ
Contact person: Ms. SCHMIDT Annette
SYNDIAL SPA - ATTIVITA DIVERSIFICATE
Contact person: Dr. TROVATO Giovanni
THE GOVERNING COUNCIL OF THE UNIVERSITY OF TORONTO
Contact person: Dr. SAVCHENKO Alexei
Contact person: Mr. FUCHS Gerhard